Polymerase chain reaction pcr principle procedure components types and applications by editorial team on january 27 2019 in microbiology virology the polymerase chain reaction pcr is a laboratory technique for dna replication that allows a target dna sequence to be selectively amplified. Polymerase chain reaction pcr is a technique used to exponentially amplify a specific target dna sequence allowing for the isolation sequencing or cloning of a single sequence among many pcr was developed in 1983 by kary mullis who received a nobel prize in chemistry in 1993 for his invention. The polymerase chain reaction and its expanding numbers of modifications have become a mainstay in diagnostic and research medicine the technique allows amplification of nucleic acid sequences both for the purposes of disease and pathogen detection and also for the preparation of hybridization probes and sequencing templates. This article throws light upon the top six applications of polymerase chain reaction the top six applications are 1 pcr in clinical diagnosis 2 pcr in dna sequencing 3 pcr in gene manipulation and expression studies 4 pcr in comparative studies of genomes 5 pcr in forensic medicine and 6 pcr in comparison with gene cloning. The pcr technique and its several advanced variants act as powerful tools which enable a multitude of specialized applications which were once deemed impossible by the scientific world
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